RESUMO: Objetivou-se avaliar o consumo e a digestibilidade aparente de ovinos alimentados com silagens de capim marandu aditivadas com resíduo de cervejaria desidratado. Foram utilizados 20 ovinos mestiços de Santa Inês machos inteiros, com peso médio inicial de 30±4.5 kg e média de 14±2meses de idade. O delineamento experimental foi o inteiramente casualizado, com cinco tratamentos e quatro repetições, sendo os tratamentos níveis de inclusão de 0, 10, 20, 30 e 40% da matéria natural de resíduo de cervejaria desidratado naturalmente por 36 horas à silagem de capim-marandu. O período de avaliação teve duração de 21 dias, sendo 15 para adaptação às dietas e seis para as coletas. Os dados foram submetidas à análise de variância, e quando significativo, as médias dos tratamentos à análise de regressão. A inclusão do resíduo de cervejaria desidratado promoveu aumento linear (P<0,05) no consumo de água, que aumentou em 0,02 ponto percentual a cada unidade de resíduo adicionada. Observou-se efeito quadrático (P<0,05) nos níveis de resíduo de cervejaria desidratado sobre o consumo de matéria seca, estimando-se consumo máximo de 2,86% peso vivo (PV) para silagens contendo 17,8% de resíduo de cervejaria. O consumo de proteína bruta apresentou comportamento quadrático, de modo que o valor máximo foi estimado em 0,29%PV de proteína bruta para o nível de 22,5% de resíduo de cervejaria desidratado. Para o consumo de extrato etéreo, houve comportamento quadrático (P<0,05), em que o valor máximo foi estimado em 0,2%PV de extrato etéreo para o nível de 28,25% de resíduo de cervejaria desidratado. O estudo de regressão mostrou comportamento quadrático (P<0,05) para o consumo de fibra em detergente neutro, em que o valor máximo foi estimado em 2,33% PV de fibra em detergente neutro para o nível de 34,58% de resíduo de cervejaria desidratado. Para os consumos de fibra em detergente ácido e matéria orgânica, não foi observada significância estatística (P>0,05). Conclui-se que silagens aditivada com níveis de 20 a 30% de resíduo de cervejaria desidratado, promoveram respostas positivas aos consumos de matéria seca, proteína bruta, extrato etéreo e fibra em detergente neutro.

ABSTRACT: The objective was to evaluate the intake and the apparent digestibility in sheep fed on marandu grass silages added with dehydrated barley. Twenty crossbred Santa Inês lambs were used, with a mean initial weight of 30±4.5 kg and mean age of 14±2 months. The experimental sheep were distributed into a completely randomized design and lining, with five treatments and four repetitions, with treatments levels of inclusion of 0, 10, 20, 30 and 40% natural matter of naturally dehydrated brewery residue for 36 hours to the marandu grass silage to feed the sheep. The evaluation period lasted 21 days, 15 for adaptation to the diets and 7 days for data collection. The data was submitted to analysis of variance, and when significant (P<0.05) the treatment means were analysed by regression. The inclusion of dehydrated barley to silage showed a linear increase in water intake (P<0.05), increased by 0.02% per unit of dehydrated barley. A quadratic effect was observed for the levels of dehydrated barley on the dry matter intake (DM), estimating a maximum consumption of 2.86% DM for silages containing 17.8% dehydrated barley. The crude protein intake (CP) behaved quadratically, with the maximum value estimated of 0.29% CP at a level of 22.5% dehydrated barley. Ether extract (EE) intake also exhibited quadratic behaviour (P<0.05), with the maximum value estimated of 0.2% EE at a level of 28.25% dehydrated barley. The regression study showed quadratic behaviour; with the maximum estimated value of neutral detergent fibre was 2.33% at a level of 34.58% dehydrated barley. No statistical significance was found for the intake of acid detergent fibre or organic matter (P>0.05). It was concluded that the addition of levels of 20 to 30% of dehydrated barley to silage resulted in a positive response for the dry matter intake, crude protein, Ether extract and neutral detergent fibre.

A method for Cr(VI) speciation in synthetic saliva after releasing from orthodontic brackets, using silica nanoparticles organofunctionalized with (3-aminopropyl)triethoxysilane (APTES) for Cr(III)/Cr(VI) separation and GF AAS determination is proposed. Under the optimized conditions, Cr(VI) speciation was performed using 150 mg of silica organofunctionalized with 2.0% (v v-1) of APTES at pH 8. It was observed different sensitivity when calibrations of GF AAS were performed using Cr(III) or Cr(VI) as standard solutions. Consequently, calibrations using stoichiometric mixtures (Cr(III) + Cr(VI)) were used for total Cr determination and calibration using Cr(VI) was used only for the determination of this specie. The reliability of the proposed silica-APTES separation procedure and GF AAS determination was checked by addition of both species in synthetic saliva. Recoveries ranging from 97 to 110% were obtained. The repeatability, based on the relative standard deviation (RSD) inter days was less than 6%. A corrosion test was carried out on 20 orthodontic brackets from two different models, after immersion in synthetic saliva (pH=6.0) at 37 °C with agitation (125 rpm) for 24 h. It was observed that about 40% of the total chromium released from the analyzed orthodontic brackets was Cr(VI).

This study measured the kinetic parameters of the ruminal degradation of the silage of Brachiaria brizantha cv. Marandu. The experiment had a completely randomized design with five treatments and five repetitions; the treatments featured the inclusión of natural brewer’s grain to Marandu grass silage at levels of 0, 10, 20, 30 and 40%. The regression equation for the degradability of the soluble Fraction A of dry matter showed a linearly increasing pattern (P<0.05) with an addition of 0.19% for each 1% of brewer’s grain included. The percentage of insoluble potentially degradable fraction (B) of dry matter was reduced linearly (P<0.05). The ruminal degradation rate in the dry matter (c), expressed as % h-1, increased linearly (P<0.05). The crude protein percentage in the Fraction B demonstrated a linear regression pattern as brewer’s grains were added (P<0.05), and there was a 0.06% reduction per unit of additive to Marandu grass silage. There was a linear increase in degradation rate c in the crude protein (P<0.05) as the additive was included in the silage. A linear increase (P<0.05) was observed for the regression of the potentially degradable fraction of NDF, represented by Fraction D. The inclusion of 20% of brewer’s grain to Marandu grass silage resulted in an improvement in the kinetic parameters of ruminal degradation.

Este estudio tuvo como objetivo medir los parámetros cinéticos de la degradación ruminal de ensilaje de Brachiaria brizantha cv. Marandu. El experimento se realizó en un diseño completamente al azar con cinco tratamientos y cinco repeticiones, los tratamientos se incluyen granos de cervecería a Marandu ensilado de hierba a niveles de 0, 10, 20, 30 y 40%. Observando la ecuación de regresión de la degradabilidad la Fracción soluble A de la materia seca, había una creciente comportamiento lineal (P<0,05), un aumento de 0,19 unidades porcentuales con la adición de 1% de la fábrica de cerveza en grano ensilado Marandu. El porcentaje de la fracción insoluble potencialmente degradable (B) de la materia seca reducida linealmente (P<0,05). La tasa de degradación ruminal (c), expresada en % h-1 el aumento linealmente (P<0,05). El porcentaje de proteína cruda en la fracción B demuestra un patrón de regresión lineal con la adición de granos de cerveza (P<0,05), hubo una reducción de 0,06% por unidad de aditivo incluido en Marandu ensilado de hierba. Para C velocidad de degradación de proteína cruda, una respuesta lineal (P<0,05), debido a la inclusión de niveles de aditivos en el ensilaje. Hubo aumento lineal (P<0,05) de la ecuación de regresión de potencialmente degradable NDF, representado por la fracción D. La inclusión de 20% de grano de cerveza a Marandu ensilado de hierba resultó en una mejora de los parámetros cinéticos de la degradación ruminal.